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superscript cdna synthesis kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript cdna synthesis kit
    Superscript Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+cdna+kit/Taq+DNA+Polymerase+Kit/pmc12892918-202-15-19
    Average 99 stars, based on 1 article reviews
    superscript cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Bovine Satellite Cells Isolated after 2 and 5 Days of Tissue Storage Maintain the Proliferative and Myogenic Capacity Needed for Cultured Meat Production
    Article Snippet: .. Reverse transcriptase was performed on 200 ng total RNA template using the SuperScript cDNA kit (Thermo Scientific) according to the manufacturer’s protocol. .. The cDNA was diluted 1:5 with RNase-free water before q-PCR analysis in technical triplicates was performed using TaqMan Universal PCR Master Mix (Thermo Scientific) according to previously described protocol [ ].

    Article Title: Adipose tissue-derived mesenchymal stem cells and keratinocytes co-culture on gelatin/chitosan/β-glycerol phosphate nanoscaffold in skin regeneration.
    Article Snippet: Using cell-based engineered skin is an emerging strategy for treating difficult-to-heal wounds To date, much endeavor has been devoted to the -fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound.. The aim of this research was to assess the impact of adipose tissue-derived mesenchymal stem cells (AD-MSCs) and keratinocytes on Gelatin/Chitosan/β-Glycerol Phosphate (GCGP) nanoscaffold in full-thickness excisional skin wound healing of rats. . For this purpose, AD-MSCs and keratinocytes were isolated from rats and GCGP nanoscaffolds were electros-pun.. Through an in vivo study, the percentage of wound closure was assessed on days 7, 14, and 21 after wound induction.

    Article Title: Eomes Expression Defines Group 1 Innate Lymphoid Cells During Metastasis in Human and Mouse
    Article Snippet: .. The RNA pellet obtained thereafter was re-suspended in water and reverse-transcribed using superscript cDNA kit (ThermoFisher). .. The cDNA obtained was diluted 5 times and qRT-PCR using Promega master-mix was performed.

    Generated:

    Article Title: CircSMAD2 accelerates endometrial cancer cell proliferation and metastasis by regulating the miR-1277-5p/MFGE8 axis
    Article Snippet: Then cell transfection was carried our using Lipofectamine 2000 (Invitrogen). .. cDNA was generated using SuperScript cDNA Kit (Invitrogen). qRT-PCR was performed in PCR System with SYBR Green (Roche Diagnostics, Burgess Hill, UK). ..

    Article Title: Twist1 Directly Regulates Genes That Promote Cell Proliferation and Migration in Developing Heart Valves
    Article Snippet: .. RNA was isolated from MC3T3-E1 cells at 80–90% confluency cultured in a 100 mm tissue culture dish (Fisher) using Trizol Reagent , . cDNA was generated using Superscript cDNA kit (Invitrogen) with 800 ng of RNA. qPCR was performed as previously described . ..

    Article Title: Chlorogenic acid attenuates hydrogen peroxide-induced oxidative stress in lens epithelial cells
    Article Snippet: .. Total RNA was extracted with TRIzol reagent (Life Technologies; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and cDNA was generated using a Superscript cDNA kit (Thermo Fisher Scientific, Inc.) based on the manufacturer's protocol. .. Following quantification with a microspectrophotometer (Beijing Kaiao Technology Development Co., Ltd., Beijing, China), cDNA was synthesized using total RNA. qPCR was performed with SYBR-Green Master Mix (Takara Biotechnology Co., Ltd., Dalian, China) in a Stratagene Mx3000P sequence detection system (Agilent Technologies, Inc., Santa Clara, CA, USA).

    Quantitative RT-PCR:

    Article Title: CircSMAD2 accelerates endometrial cancer cell proliferation and metastasis by regulating the miR-1277-5p/MFGE8 axis
    Article Snippet: Then cell transfection was carried our using Lipofectamine 2000 (Invitrogen). .. cDNA was generated using SuperScript cDNA Kit (Invitrogen). qRT-PCR was performed in PCR System with SYBR Green (Roche Diagnostics, Burgess Hill, UK). ..

    Polymerase Chain Reaction:

    Article Title: CircSMAD2 accelerates endometrial cancer cell proliferation and metastasis by regulating the miR-1277-5p/MFGE8 axis
    Article Snippet: Then cell transfection was carried our using Lipofectamine 2000 (Invitrogen). .. cDNA was generated using SuperScript cDNA Kit (Invitrogen). qRT-PCR was performed in PCR System with SYBR Green (Roche Diagnostics, Burgess Hill, UK). ..

    Article Title: Adipose tissue-derived mesenchymal stem cells and keratinocytes co-culture on gelatin/chitosan/β-glycerol phosphate nanoscaffold in skin regeneration.
    Article Snippet: Using cell-based engineered skin is an emerging strategy for treating difficult-to-heal wounds To date, much endeavor has been devoted to the -fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound.. The aim of this research was to assess the impact of adipose tissue-derived mesenchymal stem cells (AD-MSCs) and keratinocytes on Gelatin/Chitosan/β-Glycerol Phosphate (GCGP) nanoscaffold in full-thickness excisional skin wound healing of rats. . For this purpose, AD-MSCs and keratinocytes were isolated from rats and GCGP nanoscaffolds were electros-pun.. Through an in vivo study, the percentage of wound closure was assessed on days 7, 14, and 21 after wound induction.

    SYBR Green Assay:

    Article Title: CircSMAD2 accelerates endometrial cancer cell proliferation and metastasis by regulating the miR-1277-5p/MFGE8 axis
    Article Snippet: Then cell transfection was carried our using Lipofectamine 2000 (Invitrogen). .. cDNA was generated using SuperScript cDNA Kit (Invitrogen). qRT-PCR was performed in PCR System with SYBR Green (Roche Diagnostics, Burgess Hill, UK). ..

    Isolation:

    Article Title: Twist1 Directly Regulates Genes That Promote Cell Proliferation and Migration in Developing Heart Valves
    Article Snippet: .. RNA was isolated from MC3T3-E1 cells at 80–90% confluency cultured in a 100 mm tissue culture dish (Fisher) using Trizol Reagent , . cDNA was generated using Superscript cDNA kit (Invitrogen) with 800 ng of RNA. qPCR was performed as previously described . ..

    Cell Culture:

    Article Title: Twist1 Directly Regulates Genes That Promote Cell Proliferation and Migration in Developing Heart Valves
    Article Snippet: .. RNA was isolated from MC3T3-E1 cells at 80–90% confluency cultured in a 100 mm tissue culture dish (Fisher) using Trizol Reagent , . cDNA was generated using Superscript cDNA kit (Invitrogen) with 800 ng of RNA. qPCR was performed as previously described . ..

    Real-time Polymerase Chain Reaction:

    Article Title: Twist1 Directly Regulates Genes That Promote Cell Proliferation and Migration in Developing Heart Valves
    Article Snippet: .. RNA was isolated from MC3T3-E1 cells at 80–90% confluency cultured in a 100 mm tissue culture dish (Fisher) using Trizol Reagent , . cDNA was generated using Superscript cDNA kit (Invitrogen) with 800 ng of RNA. qPCR was performed as previously described . ..

    Amplification:

    Article Title: Adipose tissue-derived mesenchymal stem cells and keratinocytes co-culture on gelatin/chitosan/β-glycerol phosphate nanoscaffold in skin regeneration.
    Article Snippet: Using cell-based engineered skin is an emerging strategy for treating difficult-to-heal wounds To date, much endeavor has been devoted to the -fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound.. The aim of this research was to assess the impact of adipose tissue-derived mesenchymal stem cells (AD-MSCs) and keratinocytes on Gelatin/Chitosan/β-Glycerol Phosphate (GCGP) nanoscaffold in full-thickness excisional skin wound healing of rats. . For this purpose, AD-MSCs and keratinocytes were isolated from rats and GCGP nanoscaffolds were electros-pun.. Through an in vivo study, the percentage of wound closure was assessed on days 7, 14, and 21 after wound induction.

    Synthesized:

    Article Title: Coordinate Activation of Redox-Dependent ASK1/TGF-β Signaling by a Multiprotein Complex (MPK38, ASK1, SMADs, ZPR9, and TRX) Improves Glucose and Lipid Metabolism in Mice.
    Article Snippet: Page 1 of 65 A nt io xi da nt s & R ed ox S ig na lin g C oo rd in at e A ct iv at io n of R ed ox -d ep en de nt A SK 1/ T G FS ig na lin g by a M ul tipr ot ei n C om pl ex ( M PK 38 , A SK 1, S M A D s, Z PR 9, a nd T R X ) Im pr ov es G lu co se a nd L ip id M et ab ol is m in M ic e (d oi : 1 0.. 10 89 /a rs .2 01 5.. 63 25 ) T hi s ar tic le h as b ee n pe er -r ev ie w ed a nd a cc ep te d fo r pu bl ic at io n, b ut h as y et to u nd er go c op ye di tin g an d pr oo f co rr ec tio n. T he f in al p ub lis he d ve rs io n m ay d if fe r fr om th is p ro of .



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    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control